The chiral separation of ofloxacin racemate by ligand exchange chromatography was studied and method was\ndeveloped. A kromstar ODS column was used with the mobile phase consisting of a methanolââ?¬â??water solution (containing\ndifferent concentrations of L-isoleucine and copper sulfate as chiral mobile phase additives) at flow rate of 1 ml/min. The effect\nof different mobile phase parameters such as concentrations of chiral selector, bivalent copper ions, organic modifier and\ntemperature and flow rate on enantio separation was evaluated; the results showed that effect of different mobile phase\nparameters was strongly inter dependent. Under the optimized conditions (methanol/ water 13:87 v/v, containing 6 mmol/dm3\nL-isoleucine and Cu++ at 1.5 mmol/dm3), baseline resolution of the ofloxacin enantiomers was obtained with resolution of 1.65\nin less than 15 min.
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